We give a brief overview of SARS-CoV-2 biology, Addgene’s COVID-related plasmid resources, and how Addgene is working with the community to quickly and reliably get materials and information out to scientists that need them. Addgene is open, and dedicated to getting scientists the resources they need. For more check out Addgene’s COVID-19 Resources: https://www.addgene.org/collections/covid-19-resources/
Special thanks to Melina Fan, PhD (Co-founder & Chief Scientific Officer), Marcy Patrick (Director of Quality Control & Quality Assurance), and Caroline LaManna (Director of Marketing & Communications) for their presentation, and a thank you to all the Addgenies for their hard work and commitment these past few months.
Citations & Resources:
General references:
WHO: https://www.who.int/
CDC: https://www.cdc.gov/
NextStrain: https://nextstrain.org/
Vox: “The life-and-death consequences of naming the coronavirus”:
https://www.vox.com/2020/2/14/21135208/coronavirus-wuhan-china-covid-19-name-sars-cov-2
Open for outbreaks. Nat Biotechnol 38, 377 (2020). https://doi.org/10.1038/s41587-020-0499-y
SHERLOCK and DETECTR:
https://www.broadinstitute.org/news/enabling-coronavirus-detection-using-crispr-cas13-open-access-sherlock-research-protocols-and
https://mammoth.bio/wp-content/uploads/2020/03/Mammoth-Biosciences-A-protocol-for-rapid-detection-of-SARS-CoV-2-using-CRISPR-diagnostics-DETECTR.pdf
Ginkgo collection page: https://www.addgene.org/depositor-collections/ginkgobioworks-covid-19/
Patrick Boyle, Ginkgo Bioworks. Synthesizing DNA to fight coronavirus: https://www.ginkgobioworks.com/2020/03/20/synthesizing-dna-to-fight-coronavirus/
COVID-19 research papers and plasmids:
Structural basis of receptor recognition by SARS-CoV-2. Shang J et al. Nature. 2020, 10.1038
Paper: https://www.nature.com/articles/s41586-020-2179-y
Plasmids: https://www.addgene.org/browse/article/28211168/
A Flexible Genome-Scale Resource of SARS-CoV-2 Coding Sequence Clones. Kim, D et al. Preprints 2020, 2020040009
Paper: https://www.preprints.org/manuscript/202004.0009/v1
Plasmids:https://www.addgene.org/browse/article/28211117/
The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. Procko E. bioRxiv 2020.03.16.994236
Paper: https://www.biorxiv.org/content/10.1101/2020.03.16.994236v2
Plasmids: https://www.addgene.org/browse/article/28211097/
A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. Gordon, DE et al. bioRxiv 2020.03.22.002386
Paper: https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3
Plasmids: https://www.addgene.org/browse/article/28211149/
A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv 2020.03.29.013342
Paper: https://www.biorxiv.org/content/10.1101/2020.03.29.013342v3
Ou, X., Liu, Y., Lei, X. et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun 11, 1620 (2020). https://doi.org/10.1038/s41467-020-15562-9
Bhadra S., Maranhao A.C., Ellington A.D. A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. bioRxiv 2020.03.29.013342; doi: https://doi.org/10.1101/2020.03.29.013342
